Molecular based methods have advanced the centre testing to faster and better diagnostics. PCR methods, microarray and next generation sequencing have provided speed and
high level of accuracy. Molecular biology based methods are sensitive and quick to detect microbial pathogens in various clinical specimens.
We offer molecular detection of pathogenic viruses, bacteria and parasites from clinical specimens. MBG is ISO 15189 accredited and benefits from a stand-alone
containment Level 3 facility where samples for highly contagious pathogen are received and processed.
Rabies is a zoonotic disease that can affect all mammals. It is caused by Rabies lyssavirus, which is a neurotropic virus and is the type species of the Lyssavirus genus of the Rhabdoviridae family. These viruses are enveloped and have a single stranded negative-sense RNA genome. Rabies virus is primarily transmitted through the saliva of an infected animal. Saliva becomes infectious a few days prior to the onset of clinical signs. Infection occurs primarily via bite wounds, or infected saliva entering an open cut or wound or mucous membranes, such as those in the mouth, nasal cavity, or eyes. Occasional, albeit rare, transmission by inhalation of infected aerosol has been described.
The incubation period varies from a few days to 6 months. Clinical observations may only lead to a suspicion of rabies because signs of the disease are not pathognomonic and may vary greatly from one animal to another. The only way to undertake a reliable diagnosis is to identify the virus or viral antigen / viral RNA using laboratory tests.The disease has important social costs due to human mortality and high economic consequences due to the losses in livestock and the cost of the implementation of preventive and control measures in both animals and humans.
Method
Real-Time RT-PCR
Sample Type
Saliva, Tissue (Brain)
Transport Condition
Samples should be transported at 4°C and delivered within 24h of collection.
Specimens must be sent in RNA Preservative media. Please contact MBG Lab in advance for correct package and transport requirements.
Turn Around Time (TAT)
TAT for routine samples is within 3 working days. Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.
Acute respiratory infections present one of the most serious threats to global public health and are established to be a significant cause of morbidity and mortality in children and immune compromised adults. Most of the viruses and bacteria are the causative agents for these respiratory infections. Bacterial infections like Legionella pneumoniae, Haemophilus influenza, Mycoplasma pneumonia, Chlamydia pneumonia and Streptococcus pneumonia also viral infections such as Respiratory syncytial Virus (RSV), human influenza and Parainfluenza Virus (HPIV), human Adenovirus (HAdV), human Coronavirus (HCoV), human Rhinovirus (HRV), human Metapneumovirus (HMPV) and human Bocavirus (HBoV) are associated with a broad spectrum of symptoms such as cough, fatigue and fever.
Acute respiratory infections represent a category of infectious disease caused by multiple pathogenic agents and this increases the difficulty of diagnosis and complicates treatment strategies due to the diversity and complexity of the infectious pathogens. Rapid identification of the causative agent and timely treatment can be life saving.
Multiplex RT-PCR assays display a variety of benefits, including a significant reduction in the turnaround time of the assay compared to the use of multiple assays. Thus, this highlights the importance of establishing a rapid, effective, and accurate screening approach for the identification of causative agents of acute viral and bacterial respiratory infections.
HPP-228- Panel 2- Human parainfluenza virus 4 (HPIV-4), Human parainfluenza virus 1 (HPIV-1) Human parainfluenza virus 3 (HPIV-3)
HPP-229- Panel 3- H1N1 Influenza virus, Human Rhinovirus (HRV), Human parainfluenza virus 2 (HPIV-2)
HPP-230-Panel4-Chlamydia pneumoniae, Streptococcus pneumoniae, Mycoplasma pneumoniae all of which are bacteria
HPP-231- Panel 5-Echovirus (EV), Human bocavirus (HBoV), Parechovirus (HPeV)
HPP-232- Panel 6- Human respiratory syncytial virus A/B (RSV), Human adenovirus (HAdV), Human metapneumovirus (HMPV)
Method
Real Time RT-PCR
Sample Type
Recommended specimen types: Respiratory swab/secretion in RNA preservative media (>=3mL)
Transport Condition
Sample should be transported at 4°C.
Turn Around Time (TAT)
The Turnaround (TAT) for routine samples is within 3 working days.
Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.
Molecular based methods have advanced the centre testing to faster and better diagnostics. PCR methods, microarray and next generation sequencing have provided speed and
high level of accuracy. Molecular biology based methods are sensitive and quick to detect microbial pathogens in various clinical specimens.
We offer molecular detection of pathogenic viruses, bacteria and parasites from clinical specimens. MBG is ISO 15189 accredited and benefits from a stand-alone
containment Level 3 facility where samples for highly contagious pathogen are received and processed.
Rabies is a zoonotic disease that can affect all mammals. It is caused by Rabies lyssavirus, which is a neurotropic virus and is the type species of the Lyssavirus genus of the Rhabdoviridae family. These viruses are enveloped and have a single stranded negative-sense RNA genome. Rabies virus is primarily transmitted through the saliva of an infected animal. Saliva becomes infectious a few days prior to the onset of clinical signs. Infection occurs primarily via bite wounds, or infected saliva entering an open cut or wound or mucous membranes, such as those in the mouth, nasal cavity, or eyes. Occasional, albeit rare, transmission by inhalation of infected aerosol has been described.
The incubation period varies from a few days to 6 months. Clinical observations may only lead to a suspicion of rabies because signs of the disease are not pathognomonic and may vary greatly from one animal to another. The only way to undertake a reliable diagnosis is to identify the virus or viral antigen / viral RNA using laboratory tests.The disease has important social costs due to human mortality and high economic consequences due to the losses in livestock and the cost of the implementation of preventive and control measures in both animals and humans.
Method
Real-Time RT-PCR
Sample Type
Saliva, Tissue (Brain)
Transport Condition
Samples should be transported at 4°C and delivered within 24h of collection.
Specimens must be sent in RNA Preservative media. Please contact MBG Lab in advance for correct package and transport requirements.
Turn Around Time (TAT)
TAT for routine samples is within 3 working days. Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.
Acute respiratory infections present one of the most serious threats to global public health and are established to be a significant cause of morbidity and mortality in children and immune compromised adults. Most of the viruses and bacteria are the causative agents for these respiratory infections. Bacterial infections like Legionella pneumoniae, Haemophilus influenza, Mycoplasma pneumonia, Chlamydia pneumonia and Streptococcus pneumonia also viral infections such as Respiratory syncytial Virus (RSV), human influenza and Parainfluenza Virus (HPIV), human Adenovirus (HAdV), human Coronavirus (HCoV), human Rhinovirus (HRV), human Metapneumovirus (HMPV) and human Bocavirus (HBoV) are associated with a broad spectrum of symptoms such as cough, fatigue and fever.
Acute respiratory infections represent a category of infectious disease caused by multiple pathogenic agents and this increases the difficulty of diagnosis and complicates treatment strategies due to the diversity and complexity of the infectious pathogens. Rapid identification of the causative agent and timely treatment can be life saving.
Multiplex RT-PCR assays display a variety of benefits, including a significant reduction in the turnaround time of the assay compared to the use of multiple assays. Thus, this highlights the importance of establishing a rapid, effective, and accurate screening approach for the identification of causative agents of acute viral and bacterial respiratory infections.
HPP-228- Panel 2- Human parainfluenza virus 4 (HPIV-4), Human parainfluenza virus 1 (HPIV-1) Human parainfluenza virus 3 (HPIV-3)
HPP-229- Panel 3- H1N1 Influenza virus, Human Rhinovirus (HRV), Human parainfluenza virus 2 (HPIV-2)
HPP-230-Panel4-Chlamydia pneumoniae, Streptococcus pneumoniae, Mycoplasma pneumoniae all of which are bacteria
HPP-231- Panel 5-Echovirus (EV), Human bocavirus (HBoV), Parechovirus (HPeV)
HPP-232- Panel 6- Human respiratory syncytial virus A/B (RSV), Human adenovirus (HAdV), Human metapneumovirus (HMPV)
Method
Real Time RT-PCR
Sample Type
Recommended specimen types: Respiratory swab/secretion in RNA preservative media (>=3mL)
Transport Condition
Sample should be transported at 4°C.
Turn Around Time (TAT)
The Turnaround (TAT) for routine samples is within 3 working days.
Samples delivered after 11:00 AM will be processed next working day (unless urgent).
Urgent Samples will be reported within 24-48 hours and will be charged double.
